Department of Biology

Paul David Foglesong, Ph.D.

david

My laboratory is determining the levels of human DNA topisomerases (Topo I, Topo II a , Topo II b, Topo III a ) in normal and tumor tissures by imunohistochemistry, in situ hybridization, and catalytic assays. We have characterized over 250 tumor tissues to date. In most of the tumors there are markedly elevated levels of Topo II a and Topo II b . These results have been compared to similar studies using monoclonal antibodies against Ki-67, proliferating cell nuclear antigen (PCNA), p53 and other markers. These observations are highly relevant to chemotherapy since cells containing higher levels of Topo II are more sensitive to anti-tumor drugs which inhibit them.

We are also characterizing the effects of interleukin-2 (IL-2) upon the levels of DNA topoisomerases in HuT 78 cells (human T cell lymphoma which respond mitogenically to IL-2). Topo I activity is induced in HuT 78 cells rapidly after IL-2 treatment in three distinct peaks. The first occurs 15-30 minutes after treatment, then another peak of activity was observed after 2-4 h, and lastly, a peak was seen at 12-24 h after treatment. We have discovered that IL-2 induces the activity of Topo II in HuT 78 cells. We are characterizing the molecular mechanism(s) of the induction of Topo activity (i.e., increased transcription, translation, phosphorylation). Also, we are investigating the possible role of DNA topoisomerases in the transcription of genes following treatment of cells by IL-2.

I. Personal Data

A. Office Address: Department of Biology, CPO #311
University of the Incarnate Word
4301 Broadway
San Antonio, TX 78209-6397

B. Office Telephone: (210) 283-5033
C. Office Fax: (210) 829-3153
D. Office e-mail: davidf@uiwtx.edu

II. Academic Degrees and Postdoctoral Training

A. Postdoctoral and Research Fellow, Department of Developmental Biology
and Cancer, Albert Einstein College of Medicine, 1980-82
B. Ph.D. (Molecular Biology), Department of Microbiology, Stony Brook University, 1980
C. B.S. (Biochemistry), Department of Biochemistry and Nutrition, Virginia Tech, 1971

III. Appointments

1997- present Associate Professor of Biology, University of the Incarnate Word, San
Antonio, TX
1997-00 Member-at-Large, San Antonio Cancer Institute, San Antonio, TX
1996-97 Assistant Professor of Biology, University of the Incarnate Word, San
Antonio, TX
1989-96 Assistant Professor of Microbiology, Department of Biology, Rutgers University, Camden, NJ
1988-89 Assistant Professor, Department of Biology, Memphis State University, Memphis, TN
1986-88 Director of Biochemistry and Staff Scientist, Biochemistry Section, Biotherapeutics Inc., Memphis, TN
1982-86 Research Assistant, Department of Virology and Molecular Biology, St. Jude Children's Research Hospital, Memphis, TN
1982 National Institutes of Health Postdoctoral and Research Fellow, Department of Developmental Biology and Cancer, Albert Einstein College of Medicine, Bronx, NY

IV. Teaching Experience

A.Undergraduate Courses: General Biology, Introductory Microbiology, Cell Biology, Genetics,
General Microbiology, Immunology, Undergraduate Research in Biology
B.Graduate Courses: Microbiology, Medical Microbiology, Virology, Immunology, Molecular Biology, Recombinant DNA Techniques, Seminar in Biology, Graduate Research in Biology

V. Research Interests

A. Major Interests

1. DNA-protein interactions; eukaryotic DNA topoisomerases
2. Regulation of gene expression in eukaryotes; regulation of transcription in normal and neoplastic human cells
3. Evolution of adenoviruses and picormaviruses

B. Current Interests

1. Expression of DNA topoisomerase genes in normal and neoplastic
human tissues
2. Induction of DNA topoisomerase activity by interleukin-2
3. Inhibitors of DNA topoisomerases

VI. Professional Society Memberships

A. American Society for Biochemistry and Molecular Biology, 2003-present
B. Texas Academy of Science, 1996-present
C. Texas Branch, American Society for Microbiology, 1996-present
D. New Jersey Academy of Science, member for life since 1991
E. American Association for the Advancement of Science, 1984-present
F. New York Academy of Sciences, member for life since 1983
G. American Society for Virology, 1983-present
H. American Society for Microbiology, 1983-present

VI. Publications

A. Dissertation: Foglesong, P.D. The Purification and Characterization
of a Vaccinia Virion DNA Topoisomerase. State University
of New York at Stony Brook, pp. 1-122, 1980.
B. Selected articles:

1. Foglesong, P.D. Engagement of Students in Medically Relevant Research: Laboratory-based Scholarship of Discovery in Biology, Faculty Anthology, P.E. Lampe and J.B. Miller, eds., University of the Incarnate Word Press, submitted.

2. Foglesong, P.D. The Headwaters and Cathedral Park: Natural Laboratories for Education, Scholarship, and Conservation, pp. 77-82, The Eclectic Edition, P.E. Lampe and J.B. Miller, eds., University of the Incarnate Word Press, 2004.

3. Tovar, T. and Foglesong, P.D. DNA Topoisomerases in Breast Cancer. UIW McNair Scholars Res. J. 1: 18-21, 2000.

4. D'Andrea, M.R., Multhaupt, H.A.D., Farber, P.A., and Foglesong, P.D. Expression of Genes Encoding DNA Topoisomerase IIa and DNA Topoisomerase IIb in Human Tumors. J. Surg. Pathol. 1: 3-10, 1995.

5. D’Andrea, M.R. and Foglesong, P.D. Simultaneous Detection of Protein and mRNA for DNA Topoisomerase IIb in Human Tumors. Bull. NJ Acad. Sci. 40(2): 17-19, 1995.

6. D'Andrea, M.R., Farber, P.A., and Foglesong, P.D. Immunohistochemical Detection of DNA Topoisomerases IIa and IIb Compared to Detection of Ki-67, a Marker of Cellular Proliferation, in Human Tumors. Appl. Immunohistochem. 2: 177-185, 1994 and 3: 72, 1995.

7. D'Andrea, M.R. and Foglesong, P.D. Simultaneous Detection
of Protein and mRNA for DNA Topoisomerase IIb in Human
Tumors. Bull. NJ Acad. Sci. 40(2): 17-19, 1995.

8. Foglesong, P.D., Reckord, C., and Swink, S. Doxorubicin
Inhibits Human DNA Topoisomerase I. Cancer Chemother.
Pharmacol. 30: 123-125, 1992.

9. Foglesong, P.D. and Reckord, C. Improved Electrophoretic
Separation of Relaxed and Supercoiled DNA in the Presence
of Ethidium Bromide. BioTechniques 13: 402-404, 1992.


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